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DNA duplexes containing 5-substituted uracil pairs and N4-substituted cytosine pairs have been chemically prepared and interactions between the duplexes and metal ions have been investigated. Substitutions at the 5-position with the electron withdrawing groups, such as bromo, fluoro and cyano groups, largely alter the binding selectivity of the duplexes and the metal ions.

Metal ion binding of substituted pyrimidine base pairs in DNA duplexes