Open AccessSimultaneous use of highly acidic acridine and rigid chiral linker for efficient site-selective RNA scission
Author(s) -
YunBo Shi
Publication year - 2004
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/48.1.219
Subject(s) - linker , acridine , oligonucleotide , chemistry , rna , stereochemistry , combinatorial chemistry , acridine derivatives , bond cleavage , biochemistry , dna , organic chemistry , catalysis , gene , computer science , operating system
To the middle of oligonucleotide, 9-amino-2-methoxy-6-nitroacridine (pKa = 8.8) and 9-amino-6-chloro-2-methoxyacridine (pKa = 10.5) were tethered through three linkers, and the abilities of these conjugates for site-selective activation of RNA (inducing site-selective scission by Lu(III)) were compared. The RNA-activating ability was strongly dependent on both the acidity of acridine and the structure of linker. Combination of highly acidic acridine and rigid chiral linker leads to unprecedented efficient site-selective RNA activation.
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