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Discrimination of mismatched base pairs having various flanking sequence from normal Watson-Crick base pairs were examined using SPR sensor surface where mismatch binding ligand (MBL) were immobilized. CC, CT, and TT mismatched base pair are distinguishable from wild type DNA by means of diaminonaphthyndine dimer (damND) immobilized sensor surface. By comparing the component ratio of responses obtained by MLB immobilized sensors, distinct differences could be observed between mismatched base pairs having almost the same total SPR response.

SPR fingerprinting of mismatched base pair