Open AccessElectrochemical gene detection by using adamanthyl naphthalene diimide coupled with ferrocenyl- -cyclodextrin
Author(s) -
Shinichi Sato
Publication year - 2004
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/48.1.103
Subject(s) - ferrocene , diimide , cyclodextrin , electrochemistry , electrode , intercalation (chemistry) , dna , threading (protein sequence) , chemistry , combinatorial chemistry , ternary operation , perylene , polymer chemistry , crystallography , inorganic chemistry , organic chemistry , molecule , biochemistry , protein structure , computer science , programming language
The adamantyl moieties of adamantylnaphthalene diimide (AND) can be incorporated into the cavity of ferrocenyl-beta-cyclodextrin even where they are bound to double stranded DNA by threading intercalation. When this ternary complex is formed on the DNA probe-immobilized electrode, electrochemical DNA detection is feasible: the electrode hybridized with target DNA gave rise to a current peak corresponding to the ferrocene oxidation upon treatment with AND and ferrocenyl-beta-cyclodextrin (Fc-beta-CD).
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