Open AccessIn vitro selection and analysis of RNA aptamer recognize arginine-rich motif (ARM) model peptide on a QCM
Author(s) -
Shinobu Fukusho,
Hiroyuki Furusawa,
Yoshio Okahata
Publication year - 2000
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/44.1.187
Subject(s) - quartz crystal microbalance , peptide , rna , aptamer , systematic evolution of ligands by exponential enrichment , in vitro , chemistry , microbiology and biotechnology , biophysics , computational biology , biology , biochemistry , gene , adsorption , organic chemistry
To study RNA-peptide interactions, we performed an in vitro selection of RNA on a 27 MHz quartz-crystal microbalance (QCM) on which a simple R5 helix peptide was immobilized as a model of N peptide from bacteriophade lambda. The consensus sequences including a GNRA tetraloop were obtained from a random RNA pool after the 7th cycle selection.
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