Open AccessGC->CG transversion mutation might be caused by 8-oxoguanine oxidation product
Author(s) -
Katsuhito Kino,
Hitoshi Sugiyama
Publication year - 2000
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/44.1.139
Subject(s) - transversion , chemistry , irradiation , oxidative phosphorylation , oligomer , nuclear chemistry , photochemistry , medicinal chemistry , microbiology and biotechnology , biochemistry , mutation , organic chemistry , biology , gene , physics , nuclear physics
Oxidation of d(A6GGA6)/d(T6CCT6) with sensitizer under different UV irradiation periods was examined. Enzymatic digestion of photolysate revealed that the amount of 8-oxoG initially increased with decrease of G and then decreased with the formation of Iz. In order to investigate whether Iz was formed by one electron oxidation of 8-oxoG through long-range hole migration, photoirradiation of anthraquinone-linked oligomer was examined. The results clearly demonstrated that 8-oxoG was efficiently converted to Iz under irradiation conditions. These results suggest that Iz generated from 8-oxoG induces GC->CG transversion under oxidative condition.
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