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Novel nucleotide analogues 1-6 were prepared as "multi-substrate" analogue inhibitors for purine nucleoside phosphorylases (PNPs). The cyclopropane and the tetrahydrofuran moieties of the alkyl spacer connecting a nucleobase and difluoromethylene phosphonic acid were found to be effective for good inhibition of PNPs.

nucleic acids symposium series

Synthesis of novel nucleotide analogues possessing a difuluoromethylenephosphonato group. Evaluation of the inhibitory activity for purine nucleoside phosphorylase