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The Michaelis constant (Km) for double-stranded DNA, single-stranded DNA, and dinucleotide hydrolysis by Ce(IV) ion are 4.4, 15, and more than 40 mM, respectively. The order of the k(cat), however, is dinucleotide &gt;&gt; oligonucleotides. Not only the improvement of k(cat) but also that of Km is important for the design of an efficient artificial nuclease.

Improvement of efficiency of the Ce(IV)-induced DNA scission -- Relationship between the kinetic parameters (kcat and Km) and the DNA structure-