Open AccessImprovement of efficiency of the Ce(IV)-induced DNA scission -- Relationship between the kinetic parameters (kcat and Km) and the DNA structure-
Author(s) -
Tomoyuki Igawa,
Jun Sumaoka,
Makoto Komiyama
Publication year - 1999
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/42.1.231
Subject(s) - dna , nuclease , oligonucleotide , enzyme kinetics , kinetic energy , kinetics , chemistry , bond cleavage , michaelis–menten kinetics , hydrolysis , ion , stereochemistry , biophysics , enzyme , biochemistry , biology , catalysis , physics , organic chemistry , enzyme assay , active site , quantum mechanics
The Michaelis constant (Km) for double-stranded DNA, single-stranded DNA, and dinucleotide hydrolysis by Ce(IV) ion are 4.4, 15, and more than 40 mM, respectively. The order of the k(cat), however, is dinucleotide >> oligonucleotides. Not only the improvement of k(cat) but also that of Km is important for the design of an efficient artificial nuclease.
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