Open AccessImmobilization of DNA by UV irradiation and its utilization as functional materials
Author(s) -
M. Yamada,
Kosuke Kato,
K Shindo,
Motoyoshi Nomizu,
Nobuo Sakairi,
Hiroyuki Yamamoto,
Nobuyuki Nishi
Publication year - 1999
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/42.1.103
Subject(s) - dna , ethidium bromide , intercalation (chemistry) , pyrene , aqueous solution , irradiation , chemistry , nuclear chemistry , cellulose , chemical engineering , materials science , polymer chemistry , organic chemistry , biochemistry , physics , nuclear physics , engineering
The water-insoluble DNA film was successfully prepared by UV irradiation. The DNA film was stable in water. It could effectively accumulated the DNA-binding intercalating materials, such as ethidium bromide, dibenzo-p-dioxin and benzo[a]pyrene, in their aqueous solutions. On the other hand, DNA was immobilized onto nonwoven cellulose fabrics, also by the UV irradiation. The DNA immobilized cloth was found to bind silver ions. The DNA-cloth containing silver ion showed antibacterial activity. The water-insoluble DNA prepared by UV irradiation has a potential ability to serve as biomaterials for medical, engineering and environmental objects.
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