Open AccessStudy on structure of ribosomal RNA by time-resolved luminescence anisotropy analysis
Author(s) -
Takashi Sakamoto,
Atsushi Mahara,
Reiko Iwase,
Tetsuji Yamaoka,
Akira Murakami
Publication year - 2001
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/1.1.143
Subject(s) - luminescence , oligonucleotide , oligomer restriction , ribosomal rna , anisotropy , 16s ribosomal rna , chemistry , biophysics , microbiology and biotechnology , biology , materials science , dna , biochemistry , optics , physics , gene , optoelectronics
Evaluation of the folded structure of E.coli 16S-rRNA was performed using 5'-Ru(II) complex labeled oligodeoxyribonucleotide (Ru-probe) as the probe. This probe could evaluate the dynamic manner of the binding site of Ru-probe by using the time-resolved luminescence anisotropy technique. It was found that the rotational manner of Ru-probe in the presence of 16S-rRNA was markedly dependent on the oligonucleotide sequence, indicating that the microenvironments around the Ru-probes were largely different.
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