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Figure 2. H3K27me2 and H3K27me3 profiles of three Arabidopsis genes. (a) ChIP assays with antibodies specific against diand tri-methylated H3K27; superscripts ‘t’ and ‘d’ stand for the tri-, or di-methylated isoforms, respectively. The H3K27me2 and H3K27me3 levels for AG in clf are from the 5′-end of AG, shown in Figure 3a, below. White line on AG panel indicates the two lanes were not adjacent to the input lane. (I)-input sample is 15% of the immunoprecipitated DNA; (-) is negative control without antibody. Quantified bands from three independently performed ChIP assays are shown as graphs. Changes in H3K27me3 levels of wild type and clf SUP and AP1nucleosomes are insignificant (P > 0.5).

TheArabidopsishomologs of trithorax (ATX1) and enhancer of zeste (CLF) establish ‘bivalent chromatin marks’ at the silentAGAMOUSlocus