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Phage display libraries are now widely used in the cloning of antigen-specific antibody fragments either as Fab (light chain plus Fd portion of the heavy chain) or sFv (light and heavy chain variable domains linked via a polypeptide) (1-3). This report describes two rapid methods of screening clones during phage selection protocols: the first tests for antigen specificity by anti-M13 ELISA (4) and the second establishes the presence of both the heavy and light chain of Fab clones by PCR. The vector, NPC3, is derived from pComb3 (1).

Screening of phage display immunoglobulin libraries by anti-M13 ELISA and whole phage PCR