English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The division of total genomic libraies into chromosome-specific sublibraries is critical for efficient analysis and mapping of the genome. A new colony hybridization-based approach to rapidly identify chromosome-specific clones from a human genomic bacterial artificial chromosome (BAC) library (1,2) is described. The most ideal hybridization probe for identifying all of the chromosome-specific clones from a genomic library in a single hybridization would be the chromosome itself. Using flow sorted chromosome 22 DNA as a probe, which was obtained as previously described (3,4), we screened a 4x human genomic BAC library gridded onto 42 hybridization filters at 5 x 5 density using a Biomekl,000 'gndding tool (2). 100 ng of sorted DNA was labeled with [a-2P]dCTP and [a-32P]dATP by random priming and hybridized to the filters under suppressive condition (2). Chromosomes 21 and 22 are similar in size, and they both are acrocentric chromosomes carrying ribosomal repeats. Therefore we also hybridized the library filters with sorted chromosome 21 DNA probe as a control. Comparison of hybridization results from both chromosomal DNA allowed us to identify many of the non-chromosome-specific false positives that arose from cross hybridization between highly repetitive elements present in different chromosomes. The hybridization images were digitized via PhosphorImager (Molecular Dynamics) and processed using a computer program (5) that extracts the coordinate and intensity information for each colony on the filters. Due to the highly complex nature of the chromosomal probes, the hybridization suffered from background noise as well as low hybridization signal intensity. Quantitation and analysis of numerous weak signals with varying intensities necessitated the use of a detection method more sensitive than exposing to X-ray films and a computer based approach to read the hybridization images. Colonies belonging approximately to the top 5% intensity group from each filter were selected from each chromosomal hybridization. Most of the clones with strong signals in both chromosomal hybridizations tumed out to contain ribosomal and other repeats (Fig. 1). After excluding these common hits, -3300 and 3000 A

nucleic acids research

Selection of a sublibrary enriched for a chromosome from total human bacterial artificial chromosome library using DNA from flow sorted chromosomes as hybridization probes