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Source and Description: G2—2 E is a single copy 200 bp subfragment of G2 cloned into the EcoRV site of Bluescript KSII(+). Clone G2 was isolated from a library highly enriched for (TTAGGG)n-associated sequences (1). Polymorphisms: TaqI identifies a two allele polymorphism as assessed in 29 unrelated Caucasians. Allele Size No. of Chromosomes Frequency Al 900 bp 31 (0.54) A2 700 bp 27 (0.46) PstI identifies a two allele polymorphism as assessed in 34 unrelated Caucasians and 25 unrelated East Indians, respectively. There is an invariant band at 2.0 kb. on a panel of 8 unrelated individuals. Chromosomal Localisation: 10q22.1-10q24.3 by PCR-mapping using a somatic cell hybrid panel (BIOS Inc., New Haven) and hybrids containing various fragments of chromosome 10 (2). MendeUan Inheritance: Codominant segregation has been shown for the TaqI polymorphism in five and for the PstI polymorphism in four families.

A polymorphic DNA marker at the D8S131 locus