English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Transcription of the human neurotropic virus promoter, JCVE, and its regulation in glial cells are controlled by the 98 bp tandem repeats positioned between the viral early and late genes. Here, we show that a region, designated domain-D, located upstream from the 98 bp repeats functions as a transcriptional activator and increases JCVE promoter activity. Using the reporter SV40E promoter fused to the bacterial chloramphenicol acetyltransferase (CAT) gene, we demonstrate that domain-D stimulates the basal SV40E promoter activity in glial and to a lesser degree in HeLa cells. Results from gel mobility-shift assays indicate that domain-D interacts with proteins derived from glial and HeLa extracts and results in the formation of specific DNA-protein complexes. Through UV cross-linking assays, we demonstrate that these complexes have similar electrophoretic mobilities which comigrate with the 43-50 Kd proteins derived from glial and HeLa cells. These findings, together with our previous observations, imply that the JCVE control region is composed of multiple common and specific activator domains that may account for the increased expression of the promoter in glial cells. The possible role of the D-binding protein in transcription of the JCVE promoter is discussed.

nucleic acids research

Regulation of a human neurotropic virus promoter, JCV<sub>E</sub>: identification of a novel activator domain located upstream from the 98 bp enhancer promoter region

Regulation of a human neurotropic virus promoter, JCVE: identification of a novel activator domain located upstream from the 98 bp enhancer promoter region

Regulation of a human neurotropic virus promoter, JCV_E: identification of a novel activator domain located upstream from the 98 bp enhancer promoter region