English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Open

Presents the pdf analysis as premium feature

PDF Analysis

Insights

Presents the summarisation as premium feature

Summarisation

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

We have isolated and sequenced three clones from rat stomach or kidney cDNA libraries. These clones encode the cytoskeletal isoform of 7-actin as determined by comparison with published protein sequence (1), as well as with cDNA sequences from the human (2) and mouse (3). The sequence of the longest kidney clone, PRC7A-2, is shown below. It displays poly 'G' and ' C homopolymeric tails, 12 nucleotides (nt) of 5' untranslated sequence, the entire cytoskeletal 7-actin coding sequence, and 716 nt of 3' untranslated region (3'UTR) which contains a consensus polyadenylation signal and poly-A tail. Two other clones, pROyA-15 were isolated from a rat stomach signal and poly-A tail. Two other clones, PRC7A-4 and PRC7A-4 and PRC7A-I5 were isolated from a rat stomach library. These clones are identical in nucleic acid sequence to pRC>A-2 in aligned regions, but are truncated on both the 3' and 5' ends. In addition, clone PRC7A-4 contains an internal deletion of 145 nt in the 3'UTR. The deleted sequence has been underlined below. The 3' untranslated region of the rat cytoskeletal 7-actin mRNA has been well-conserved throughout evolution as demonstrated by a 94.6% and 81.5% similarity with mouse and human 3'UTR's, respectively (3, 2). There are a number of structural features in this region of the molecule which should be mentioned. First, there are two inverted repeat sequences which are predicted to be very stable, according to the rules of Tinoco et al. (4). These regions are boxed below. Also, a consensus 'TATA' transcription initiation signal is evident beginning at nt 1488. Interestingly, transcription of the human salivary a-amylase gene has been shown to initiate approximately 20 nt downstream of a comparable site in a cytoskeletal 7-actin pseudogene (5, 6). This finding demonstrates that the 3'UTR of cytoskeletal 7-actin in an altered form can serve as a functional promoter.

A cDNA sequence encoding cytoskeletal gamma-actin from rat