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Source: Description: pKM-19 is a 1.0 kb EcoRI human genomic fragment inserted in pUC13, that detects a Scrfl (CC/NGG) RFLP (1, 2). We report here the primer sequences suitable for the detection of this RFLP by PCR..

PCR detection of the pKM.19/Scrfl RFLP (D7S23), a marker closely linked to the cystic fibrosis mutation