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The mra (murein synthesis cluster a) region at 2 min on the Escherichia coli chromosome map carries the genes for peptidoglycan synthesis (1, 2): genes murC, murD, murE, murF and ddl, coding for five enzymes synthesizing UDP-Nacetylmuramyl-pentapeptide (L-alanyl-D-glutamyl-mesodiaminopimelyl-£>-alanyl-Z)-alanine) from UDP-N-acetylmuramic acid by sequential addition of appropriate amino acids. The genes pbpB and ftsW (3) that code for septum-peptidoglycan synthetic proteins, murG (4) which produces a protein of unknown function and an open reading frame ORF-Y (5) were also located in the mra region. In the present study, we determined the base sequences of the murG and murC genes located between JisW and ddl and thus completed the sequencing of the total 12 kb mra region which is flanked by a 5 kb region involving JisQ, JisA,ftsZ and envA. An open reading frame of 1065 bp capable of encoding a moderately hydrophobic peptide with 355 amino acid residues (Mw 37,814) was found for murG, and one of 1473 bp encoding a peptide with 491 amino acid residues (Mw 53,625) was found for murC. The proteins MurG and MurC were detected on SDS/PAGE in an in vitro protein synthesis system. Overlapping of the open reading frames of the murG-murC area and its flanking genes ftsWand ddl was observed. Considerable homologies were found in the deduced amino acid sequences of the product proteins of murC, D, £and F, i.e., four ligases that synthesize UDP-N-acetylmuramyl-pentapeptide, including putative ATP-binding domains GXXGKT/S (126-131 of MurC). MurG showed considerable homology with the corresponding region of the Bacillus subtilis chromosome.

Nucleotide sequence involving murG and murC in the mra gene cluster region of Escherichia coli