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. To determine the sequence of the ER gene variant region, this portion (from nucleotide 189 to 366 downstream from the initiation codon) (3) was amplified from genomic DNA obtained from three heterozygotes . Taq polymerase-catalyzed DNA amplification (5) made use of two synthetic DNA primers : ER1) 5'-CtgcaGCGCAGGTC TACGGTCAGACC-3' and ER2) 5'AGCaAGCTtCGGCGGCGGGTGCAGT-3' . (Small letters indicate bases included in the primers to generate Pstl and HindIII restriction sites at the ends of the amplified products .) Amplified products were treated with restriction

Identification of a variant form of the human estrogen receptor with an amino acid replacement