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SOURCE/DESCRIPTION: A 4.0 kb TaqI fragment of cosmid EFD64 isolated by a HBV-3 oligonucleotide (1) was subcloned into AccI site of pUC18. POLYMORPHISM: Mspl identifies 5 allelic VNTR polymorphism with bands between 2.6 and 4.6 kb. Rsal, TaqI, EcoRI, BamHI, Hindlll and Pvull identify the same VNTR polymorphism. FREQUENCY: With Rsal, 80 % heterozygosity were observed in 80 unrelated Caucasians. NOT POLYMORPHIC FOR: Bglll CHROMOSOMAL LOCALIZATION: pEFD64.2 has been assigned to chromosome 3 by linkage analysis (2) with loci (APOD) known to span this chromosome (3). MENDELIAN INHERITANCE: Co-dominant segregation of the Rsal RFLP was observed in 40 three generation families. PROBE AVAILABILITY: Freely available (contact Y.N.) or will be available from ATCC. OTHER COMMENTS: RFLPs were observed under the normal hybridization and washing condition.

Isolation and mapping of a polymorphic DNA sequence (pEFD64.1) on chromosome 3 [D3S42]