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We previously reported that O4-alkyl dTTPs could replace, for short times, dTTP in polymer synthesis [Singer et al., PNAS 83, 26-32, 1986]. The reasons for such early termination of synthesis could be either proofreading or the eventual formation of weakly paired primer termini. Utilizing the known 3'----5' exonucleolytic activity of polymerases, in the absence of dNTPs, enabled us to conclude that, in contrast to the digestibility of poly[d(A-T)] which yielded the expected 3'-mononucleotides, the polymerizing enzymes did not digest O4-methyl dT or its neighbors. The presence of the resistant alpha-phosphorothionate linkage did not prevent measurable digestion of poly[d(A-T)] by the Klenow fragment. This, together with evidence that polymerization of O4-methyl dTTP is favored at low temperatures, supports the model proposed by Ollis et al. [Nature 313, 762-766, 1985] showing independent domains for the two activities in the Klenow fragment.

nucleic acids research

O<sup>4</sup>-Methyldeoxythymidine replacing deoxythymidine in poly[d(A—T)] renders the polymer resistant to the 3′ → 5′ exonuclease activity of the Klenow and T<sub>4</sub>DNA polymerases

O4-Methyldeoxythymidine replacing deoxythymidine in poly[d(A—T)] renders the polymer resistant to the 3′ → 5′ exonuclease activity of the Klenow and T4DNA polymerases

O⁴-Methyldeoxythymidine replacing deoxythymidine in poly[d(A—T)] renders the polymer resistant to the 3′ → 5′ exonuclease activity of the Klenow and T₄DNA polymerases