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Deoxydecanucleotides having a recognition sequence of Bgl II and Sau 3AI, and their 7-deazaadenine analogs were synthesized. The decanucleotides containing 7-deazaadenine in place of adenine were partially resistant to the hydrolysis by Sau 3AI and strongly resistant to that by Bgl II. A new hypothesis on the mode of recognition and cleavage of specific nucleotide sequences by Bgl II, recognizing one strand and cleaving the other strand, is presented.

nucleic acids research

Synthesis of deoxyoligonucteotides containing 7-deazaadenine: recognition and cleavage by restriction endonuclease<i>Bgl</i>II and<i>Sau</i>3AI (Nucleosides and Nucleotides Part 551)

Synthesis of deoxyoligonucteotides containing 7-deazaadenine: recognition and cleavage by restriction endonucleaseBglII andSau3AI (Nucleosides and Nucleotides Part 551)