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In cells of E.coli K12 grown exponentially in minimal medium, tRNA(met), tRNA(leu) and tRNA(ile) are aminoacylated at 100%, 80% and 64%, respectively. On addition of L-methioninyl adenylate to the growth medium, one observes a specific deacylation of tRNA(met). When more than 35% of tRNA(met) is deacylated, growth rate is reduced and becomes proportional to the amount of methionyl-tRNA formed.

Effect of L-methioninyl adenylate on the level of aminocylation in vivo of tRNAMetEscherichia coli K12

Effect of L-methioninyl adenylate on the level of aminocylation in vivo of tRNA^MetEscherichia coli K12