English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The Random Amplified Polymorphic DNA (RAPD) assay was used to study genetic variation within and between 9 species of the genus Bulinus and to determine whether RAPD profiles could be used as markers for identification purposes. RAPDs were generated with 8 primers of two different sizes (lOmers & 15mers) and were visualised using both polyacrylamide gel electrophoresis (PAGE) with silver staining and agarose gel electrophoresis with ethidium bromide staining. The species groups of Bulinus had few similarities in their RAPD profiles and there was interspecific variation within groups. Intrapopulation variation was observed, with all primers, for B globosus collected from a single site in Zimbabwe PAGE/silver staining methods visualised a greater number of RAPDs in comparison with agarose/ethidium bromide methods. Phenetic analysis indicated that distance estimates between taxa were sometimes non-additive and the phylogenetic analysis of such non-metnc data is discussed. The resultant phenograms, constructed using a least squares method, were constrained almost into a polytomy with topologies often differing between data sets. It was concluded that this phenomenon was most likely attributable to large nucleotide divergences between the species groups which go beyond the phylogenetic scope of RAPD analysis. RAPD profiles, when used in conjunction with other taxonomic methods, may contribute to the identification of species of Buhnus on a regional basis, but the observed variability in a natural population suggests that a diagnostic RAPD profile for each species throughout its geographic range is unlikely.

journal of molluscan studies

AN EVALUATION OF RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) FOR THE IDENTIFICATION AND PHYLOGENY OF FRESHWATER SNAILS OF THE GENUS <i>BULINUS</i> (GASTROPODA: PLANORBIDAE)