Open AccessTranscription Factor ZNF326 Upregulates the Expression of ERCC1 and HDAC7 and its Clinicopathologic Significance in Glioma
Author(s) -
Minghao Wang,
Qiang Han,
Zhe Su,
Xinmiao Yu
Publication year - 2020
Publication title -
laboratory medicine
Language(s) - English
Resource type - Journals
eISSN - 1943-7730
pISSN - 0007-5027
DOI - 10.1093/labmed/lmz075
Subject(s) - downregulation and upregulation , ercc1 , gene knockdown , glioma , cancer research , transcription factor , biology , messenger rna , transcription (linguistics) , immunohistochemistry , microbiology and biotechnology , gene , immunology , genetics , nucleotide excision repair , linguistics , philosophy , dna repair
Previous reports that we have coauthored have shown that transcription factor ZNF326 can upregulate the expression of ERCC1 and HDAC7, and downregulate the expression of LTBP4 and ZNF383 in lung-cancer cells. However, whether tissue-specificity of the ZNF326 function exists in glioma tissue remains unclear. In this study, overexpression or knockdown of ZNF326 in glioma cells caused upregulation or downregulation, respectively, of the protein and micro RNA (mRNA) levels of ERCC1 and HDAC7. The levels of LTBP4 and ZNF383 were not significantly changed. Immunohistochemical results showed that ZNF326 was not only highly expressed in glioma but was also positively correlated with the expression of ERCC1 and HDAC7. Moreover, the expression of ERCC1 and HDAC7 was enhanced with the increase in tumor grade. However, there was no correlation between ZNF326 and the expression of LTBP4 and ZNF383. Therefore, the detection of ZNF326, ERCC1, and HDAC7 expressions was useful for identifying different grades of glioma.
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