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QTLs with flanking markers showing significant allele frequency differences in the BSA studies will indicate The usual method to locate and compare loci regulat- those traits likely to be important in determining yield ing quantitative traits (QTLs) requires a segregating under drought. population of plants with each one genotyped with molecular markers. However, plants from such segreg- Key words: Bulk segregant analysis (BSA), drought ating populations can also be grouped according to resistance, genetic maps, maize, molecular markers, Zea phenotypic expression of a trait and tested for differ- mays (L.). ences in allele frequency between the population bulks: bulk segregant analysis (BSA). The same probes used for making a genetic map (e.g. isozyme, RFLP, Introduction RAPD, etc) can be used for BSA. A molecular marker Improving the drought resistance of crop plants is a

Bulk segregant analysis with molecular markers and its use for improving drought resistance in maize