Open AccessDetermination of apoplastic K+in intact leaves by ratio imaging of PBFI fluorescence
Author(s) -
Karl H. Mühling,
B. Sattelmacher
Publication year - 1997
Publication title -
journal of experimental botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.616
H-Index - 242
eISSN - 1460-2431
pISSN - 0022-0957
DOI - 10.1093/jxb/48.8.1609
Subject(s) - apoplast , fluorescence , potassium , chemistry , analytical chemistry (journal) , botany , chromatography , biology , biochemistry , cell wall , physics , organic chemistry , quantum mechanics
The tetraammonium salt of the K + binding fluorescent dye benzofuran isophthalate (PBFI) was used to investigate the influence of potassium nutrition (0.1-2.1 mol m -3 ) on apoplastic K + in Vicia faba leaves by means of ratio imaging. As a reference the infiltration-centrifugation method was used. Both methods reflected the influence of K + supply on apoplastic K + concentration. The abaxial leaf side revealed significantly higher K + concentrations (20-25 mol m -3 ) than the adaxial side (5-8 mol m -3 ). Application of CCCP led to an immediate increase in apoplastic K + demonstrating the reliability of the PBFI method.
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