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We identified Naegleria fowleri as the aetiologic agent from cerebrospinal fluid of a patient with a travel history to Karachi. We demonstrate the benefit of metagenomics-based rapid diagnosis of primary amoebic meningoencephalitis when routine clinical tests fail to provide a definitive diagnosis. We reconstructed the N. fowleri genome.

Metagenomics-driven rapid diagnosis of an imported fatal case of rare amoebic meningoencephalitis