English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Brandes et al. (1) published a report in the Journal in which they claimed that Hrantihistamines, such as loratadine, astemizole, and hydroxyzine, enhanced murine tumor growth. As a formal opinion regarding this issue, Health Canada and the Food and Drug Administration (FDA) declared the necessity of confirmation of the results of Brandes et al. (1). As a result, we reassessed the effects of astemizole on the cell proliferation of murine tumors (B16F10 melanoma cells) to confirm the reproducibility of their results. However, we were not able to reproduce them (2). As a final approach, we assessed the effects of astemizole on the proliferation of two human-derived tumor cells, HMV-1 melanoma (3-5) and HT1080 fibrosarcoma (6), both in vitro and in vivo. In the in vitro studies, the number of colonies (>1 mm diameter) and bromodeoxyuridine (BrdU) uptake (7,8) were measured after a 24-hour treatment with astemizole (10~-l(T M). For in vivo studies, 7-week-old female CB-17 scid/ scid (SCID) mice (9,70) under anesthesia were given subcutaneous inoculations of cells from each solid tumor (2 x 2 x 2 mm). Tumor-bearing mice (each tumor approximately 100 mm) were randomly assigned to each of four groups and then treated orally with astemizole (6.0, 20.0, or 60.0 mg/m per day) or the vehicle control for 10 days. On the day after the final dosing, the tumors were excised and weighed. In in vitro studies, the number of colonies and the amount of BrdU uptake in astemizole-treated groups were not significantly different from those in the control group. Also, in in vivo studies, astemizole did not promote the cell growth of human HMV-1 melanoma or HT1080fibrosarcoma (Fig.l). Thus, both our earlier results on B16F10 melanoma conducted in vitro

Re: Enhanced Cancer Growth in Mice Administered Daily Human-Equivalent Doses of Some H1-Antihistamines: Predictive In Vitro Correlates