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A non-autoclaved solid diet was used to evaluate the entomopathogenic fungus  Beauveria bassiana  (Balsamo) Vuillemin (Hypocreales: Clavicipitaceae) strain NI8 and the insect growth regulator novaluron (Diamond® 0.83EC insecticide) for control of the tarnished plant bug,  Lygus lineolaris  (Palisot de Beauvois) (Hemiptera: Miridae). The diet was composed of toasted wheat germ, ground lima bean meal, soy flour, yolk of chicken eggs, inhibitor, and agar. It was prepared in one step by blending the ingredients in boiling water. The diet was used to bioassay  L. lineolaris  from the second instar to the adult stage. Fourth and fifth instars and adults of  L. lineolaris  were more susceptible than second and third instars to infection by  B. bassiana  , whereas second, third, and fourth instars had higher mortality than fifth instars 10 days after exposure to novaluron. No effects on longevity were observed in adults treated with novaluron when compared with the control, but longevity was significantly different from that of adults exposed to  B. bassiana  . Adults of  L. lineolaris  were maintained for over a month without changing the diet. The non-autoclaved diet is semi-liquid before it cools, which facilitates the mechanics of diet packaging similar to food packaging or lepidopteran diet preparation. This solid artificial diet for  Lygus  bugs provides improved research capacity for studying the ecology and susceptibility of  Lygus  spp. to a number of different control agents, including beneficial organisms, insect pathogens, and insecticidal toxins being developed for transgenic technologies.

journal of insect science

A novel bioassay to evaluate the potential of Beauveria bassiana strain NI8 and the insect growth regulator novaluron againstLygus lineolaris on a non-autoclaved solid artificial diet