Open AccessThe monoclonal antibody MAC252 does not react with the (−) enantiomer of abscisic acid
Author(s) -
Philippe Barrieu,
Thierry Simonneau
Publication year - 2000
Publication title -
journal of experimental botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.616
H-Index - 242
eISSN - 1460-2431
pISSN - 0022-0957
DOI - 10.1093/jexbot/51.343.305
Subject(s) - abscisic acid , monoclonal antibody , enantiomer , chemistry , reactivity (psychology) , monoclonal , antibody , biochemistry , stereochemistry , biology , medicine , immunology , gene , pathology , alternative medicine
An RIA procedure has been developed for ABA quantification using MAC62, a monoclonal antibody raised against (+)-cis, trans -ABA. This widely used method now relies on MAC252, a recloned version of the exhausted MAC62. Recently, it has been suggested that MAC252 was not able to discriminate between the (+) and (-) enantiomers of ABA. As this can be misleading when interpreting RIA results, it has been carefully examined here whether MAC252 reacts with (-)-ABA. MAC252 exhibited negligible cross-reactivity with (-)-ABA, which was confirmed with commercial mixtures of ABA isomers. It is concluded that the RIA protocol can continue to be used with MAC252 as it was with MAC62.
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