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A bioassay employing sweetclover root disks impregnated with various plant extracts has been developed. The bioassay has been used to demonstrate the distribution of substances influencing feeding in fractionated water-methanol-chloroform extracts of Melilotus infesta Guss. and M. officinalis L. Lam. leaves. Indications are that substances responsible for the resistance of M. officinalis a or the susceptibility of M. officinalis to feeding by adult sweetclover weevils, Sitona cylindricollis , Fahraeus, reside in the water-methanol fraction.

A Bioassay for Detecting Compounds Which Stimulate or Deter Feeding by the Sweetclover Weevil12