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A quantitative analysis was developed for the determination of cocaine, benzoylecgonine, and cocaethylene in oral fluid using liquid chromatography-tandem mass spectrometry. After internal standardization and solid-phase extraction, chromatographic separation was achieved on a reversed-phase column by gradient elution. The reconstructed mass chromatograms of the collision-induced dissociation transitions of m/z 290 --&gt; m/z 168 (benzoylecgonine), m/z 304 --&gt; m/z 168+119 (2'-methylbenzoylecgonine), m/z 304 --&gt; m/z 182 (cocaine), m/z 318 --&gt; m/z 196 (cocaethylene), and m/z 318 --&gt; m/z 182+119 (2'-methylcocaine) were used for quantitation. The developed method was adequately validated. Good linearity was obtained from 10 to 1000 microg/L. Extraction recoveries exceeded 85% for all compounds. Excellent total and within-run reproducibilities (CV% &lt; 20%) and accuracy figures were obtained. The limit of detection (signal-to-noise ratio &gt;/= 3) was 1 microg/L for all three compounds. As such, a method for drug abuse confirmation analysis in oral fluid, compatible with the present day saliva collecting devices, is obtained. The method was applied to real samples (n = 15) obtained from suspected drug users, of which seven proved positive. The concentrations found in the positive samples were between 10.2 and 200.6 microg/L for cocaine, &lt; limit of quantification (LOQ) and 10.5 microg/L for cocaethylene, and &lt; LOQ and 59.2 microg/L for benzoylecgonine.

journal of analytical toxicology.

The Determination of Cocaine, Benzoylecgonine, and Cocaethylene in Small-Volume Oral Fluid Samples by Liquid Chromatography-Quadrupole-Time-of-Flight Mass Spectrometry