Open AccessDetermination of Δ9-THC in Whole Blood using Gas Chromatography-Mass Spectrometry
Author(s) -
Mark Chu,
Olaf H. Drummer
Publication year - 2002
Publication title -
journal of analytical toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.161
H-Index - 76
eISSN - 1945-2403
pISSN - 0146-4760
DOI - 10.1093/jat/26.8.575
Subject(s) - chromatography , chemistry , derivatization , mass spectrometry , detection limit , gas chromatography , extraction (chemistry) , gas chromatography–mass spectrometry , coefficient of variation , whole blood , quantitative analysis (chemistry) , immunology , biology
A simple and reliable liquid-liquid extraction method for the determination of delta9-tetrahydrocannabinol (THC) in whole blood utilizing gas chromatography-mass spectrometry in electron impact mode is described. The substance is derivatized with pentafluoropropionic anhydride in pentafluropropanol. The limit of detection is 0.5 ng/mL for a 1-mL specimen, with recovery greater than 70%. The intra-assay coefficient of variation (CV) is 3.1% to 5.2%, and the interassay CV is 6.4% to 9.5%, calculated at THC concentrations of 1, 5, and 25 ng/mL. The accuracy is between 95 and 97%. The optimization of extraction and derivatization conditions is detailed.
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