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A method for the determination of delta9-tetrahydrocannabinol (THC) and 11-nor-9-carboxy-delta9-tetrahydrocannabinol (THCCOOH) in whole blood using conventional solvent extraction followed by a cleanup using polar solid-phase cartridges is described. The method uses 0.5 mL of blood, and detection is by benchtop gas chromatography-mass selective detection using selected ion monitoring. The limit of detection is better than 1 ng/mL, and extraction efficiencies are greater than 80% for THC and 70% for THCCOOH. The method is robust and highly reproducible.

journal of analytical toxicology.

The Determination of 9-Tetrahydrocannabinol and 11-Nor-9-Carboxy- 9-tetrahydrocannabinol in Whole Blood Using Solvent Extraction Combined with Polar Solid-Phase Extraction