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1 Definitions: Probability of detection (POD) is the proportion of positive analytical outcomes for a qualitative method for a given matrix at a given agent level or concentration. POD is concentration-dependent. The acceptable minimum detection level (AMDL) is the predetermined minimum level of a biological threat agent, which must be detected by the candidate method with an estimated 5% lower confidence limit on the POD of 0.95 or higher. The AMDL is dependent on the intended use.

journal of aoac international

AOAC SMPR 2010.003: Standard Method Performance Requirements for Polymerase Chain Reaction (PCR) Methods for Detection of Bacillus anthracis in Aerosol Collection Filters and/or Liquids