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A single-laboratory validation study was conducted for an LC method using UV detection for the simultaneous determination of the active ingredients o-phenylphenol (OPP), p-tert-amylphenol (PTAP), and o-benzyl-p-chlorophenol (OBPCP) in disinfectant formulations. Samples were extracted, the extracts diluted with acidified methanol, and the active ingredients separated by LC with a gradient mobile phase and quantified by using UV detection at 285 nm. For each active ingredient, the RSD was 3.7, and the intermediate reproducibility was 3.4. The active ingredient content of the spiked samples analyzed in this study ranged from 0.075 to 10.1 for the individual phenol active ingredients. The average recovery ranges were 86.7104.9, 82.8115.6, and 91.6114.7 for the active ingredients OPP, PTAP, and OBPCP, respectively, for the concentration range of 0.07510.1. This method, with a relatively short chromatographic run time (about 15 min), proved to be reliable and convenient for analyses of products or samples containing all or a combination of these phenol active ingredients.

journal of aoac international

Single-Laboratory Validation of a Method for the Determination of Phenols and Phenates in Disinfectant Formulations by Liquid Chromatography with UV Detection