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An efficient, sensitive, and simple method was developed for the simultaneous determination of rosiglitazone and metformin hydrochloride in a combination tablet dosage form by column high-performance liquid chromatography. The mobile phase used was ammonium dihydrogen phosphate adjusted to pH 5.25 with sodium hydroxide. The limits of detection and quantitation were in the range of 0.51.6 g/mL, respectively, for metformin hydrochloride, and 0.002010.0067 g/mL, respectively, for rosiglitazone. The linearity was studied in the concentration range of 0.120.31 g/mL for rosiglitazone and 30.676.7 g/mL for metformin hydrochloride. The recovered amounts of metformin hydrochloride and rosiglitazone were 100103.8 and 101103.7, respectively.

journal of aoac international

Column High-Performance Liquid Chromatographic Method for the Simultaneous Determination of Rosiglitazone and Metformin in a Pharmaceutical Preparation