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A reversed-phase liquid chromatography method for determination of maduramicin in feedingstuffs and premixtures was developed, validated, and interlaboratory studied. The extraction solvent was methanol. Maduramicin was detected at 520 nm after postcolumn derivatization with vanillin.

Determination of Maduramicin in Feedingstuffs and Premixtures by Liquid Chromatography: Development, Validation, and Interlaboratory Study