Open AccessDevelopment, Validation, and Standardization of Polymerase Chain Reaction-Based Detection of E. coli O157
Author(s) -
Amir Abdulmawjood,
Michael Bülte,
Stefanie Roth,
H. Schönenbrücher,
Nigel Cook,
Annet Heuvelink,
Jeffrey Hoorfar
Publication year - 2004
Publication title -
journal of aoac international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 87
eISSN - 1944-7922
pISSN - 1060-3271
DOI - 10.1093/jaoac/87.3.596
Subject(s) - polymerase chain reaction , standardization , microbiology and biotechnology , escherichia coli , chemistry , gene , biology , chromatography , genetics , computer science , operating system
A diagnostic polymerase chain reaction assay was developed for the detection of E. coli O157 as the first part of a multicenter validation and standardization project. The assay is based on amplification of sequences of the rfbE O157 gene and includes an internal amplification control. The selectivity of the assay was evaluated against 155 strains, including 32 E. coli O157, 38 E. coli non-O157, and 85 non-E. coli. It was shown to be highly inclusive (100%) and exclusive (100%). The assay has a 100% detection probability of approximately 2 × 103 cells per reaction.
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