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A method was developed for the simultaneous determination of imazalil and its major metabolite, R 14821, in citrus fruit. This method (designated the SPE method) is based on solid-phase extraction (SPE) and capillary gas chromatography (GC) with electron-capture detection (ECD). The SPE method is highly sensitive for both compounds (limit of detection for each: 0.001 microg/g) and is less time consuming than the previously reported method based on repeated liquid-liquid partitioning and GC-ECD. Recoveries for 3 levels of fortification (0.02, 0.2, and 2.5 microg/g) from satsuma mandarins ranged from 94.3 to 96.5% for imazalil and from 93.9 to 96.3 % for R 14821, with coefficients of variation (CVs) ranging from 3.1 to 6.3% for imazalil and from 4.5 to 5.6% for R 14821. Average residual levels found in grapefruit, oranges, and lemons by the SPE method and the previously reported method, and the corresponding CVs, were similar.

journal of aoac international

Simultaneous Determination of Imazalil and Its Major Metabolite in Citrus Fruit by Solid-Phase Extraction and Capillary Gas Chromatography with Electron Capture Detection