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This study is the first Raman spectroscopic characterization of conventional chemically bonded liquid chromatographic (LC) stationary phases of the RP-18 type using the high-power Nd:WV04 neodymium laser. Raman spectra were obtained for octadecyl (C18) chemically bonded silica-based stationary phases, as well as for silica, aminopropyl (NH2), cyanopropyl (CN), diol (OH), octyl (C8), and methyl (C1) phases for comparison. This study demonstrates that Raman spectroscopy using the high-power neodymium laser can be used to study RP-18-type LC stationary phases and to quantitate the density of coverage of the silica matrix with octadecyl ligands. The paper presents the experimental setup and conditions usedto obtain Raman spectra of commercial RP-18-type chemically bonded stationary phases and examines the spectroscopic similarities and differences observed for different bonded ligands typically used in reversed-phase and normal-phase LC separations.

Raman Spectroscopic Characterization of RP-18-Type Chemically Bonded Stationary Phases for Liquid Chromatography