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Studies were initiated to determine whether tetracycline components such as tetracycline and 4-epichlortetracycline present in commercial feed grade chlorteracycline (CTC) products interfere with AOAC Method 967.39. These investigations confirmed that the microbiological result for feed grade concentrates and premix products is not affected by the biomass matrix containing low levels of these other tetracycline components. Thus, the microbiological result can be directly correlated to the single CTC peak response in liquid chromatographic analytical procedures.

Investigation of Assay Interference with Microbiological Determinations of Chlortetracycline in Feed Grade and Premix Samples