Open AccessMechanisms for Mucosal Immunogenicity and Adjuvancy of Escherichia coli Labile Enterotoxin
Author(s) -
Ichiro Takahashi,
Mariarosaria Marinaro,
Hiroshi Kiyono,
R J Jackson,
Ichirô Nakagawa,
Keiko Fujihashi,
Shigeyuki Hamada,
John D. Clements,
Kenneth L. Bost,
Jerry R. McGhee
Publication year - 1996
Publication title -
the journal of infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.69
H-Index - 252
eISSN - 1537-6613
pISSN - 0022-1899
DOI - 10.1093/infdis/173.3.627
Subject(s) - immunogen , immunogenicity , toxoid , enterotoxin , antibody , heat labile enterotoxin , escherichia coli , microbiology and biotechnology , biology , enterotoxigenic escherichia coli , adjuvant , immunology , spleen , immunoglobulin a , immunoglobulin g , monoclonal antibody , immunization , biochemistry , gene
Escherichia coli labile toxin (LT) was assessed as mucosal immunogen and as adjuvant for tetanus toxoid (TT) in mice. After oral administration of LT, C57BL/6 (H-2b) and BALB/c(H-2d) mice were high mucosal and serum antibody responders, while C3H/HeN (H-2k) mice were low responders. High responders exhibited mainly serum IgG (including IgG1, IgG2a, and IgG2b), as well as IgM and IgA, while mucosal responses were IgA. Analysis of LT-B-specific CD4+ T helper (Th) cells from Peyer's patches (PP) or from spleen revealed a mixed Th1 (interferon-gamma) and Th2 (interleukin-4 and -5) cell pattern. Oral LT given with TT induced TT-specific response patterns identical to LT-B. Analysis of mRNA from TT-specific PP CD4+ Th cells also revealed a mixed Th1- and Th2- type response. Thus, antibody response profiles induced by LT are regulated by both CD4+ Th1 and Th2 cell types.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom