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Strawberry sap beetle adults were laboratory-reared and maintained on autoclaves prunes at 23 ± 2°C and 70% RH under a 16-hr photoperiod. Stock solutions (0.01%) of each insecticide tested were prepared from technical grade materials and diluted with technical grade acetone. Two replicates of 20beetles each (mixed sex and age) were anesthetized by placing them in a 9.0-cm plastic petri dish and placing the dish on a cold surface (ca. 1.0° C). Topical applications (1 μl/beetle) were applied to the venter of the abdomen with a 0.25-cc glass syringe using a microapplicator. After treatment, beetles were placed in inverted 9.0-cm plastic petri dishes lined with filter paper discs. A 10% honey solution was added to the filter paper via a plastic squeeze bottle. The honey solution provided a carbohydrate source as well as water. Controls consisted of 2 replicates which were treated with the solvent and 2 replicates which were not treated. All beetles were placed in an insect-rearing room maintained under the above conditions. Mortality was assessed 24 hr after treatment.

Topical Toxicity of Insecticides to Strawberry Sap Beetle Adults, Ohio, 1978