Mouse-Cuterebra Animal Systemic Insecticide Test, 1976

White mice are infested ocularly with 5 newly hatched larvae of Cuterebrafontinella. Two days later, female mice are weighed and treated orally (mg/kg basis) with candidate insecticides formulated in Tween 20; male mice have a plastic collar placed around their necks (to prevent grooming) and are dipped (body only) into 100-200 ml of an emulsion of the candidate insecticide usually formulated as an EC in xylene (65 parts), Triton X-100 (10 parts), and Al (25 parts). Highest dosages are 100 mg/kg and 1%. Four days later, mice are killed, and bodies examined carefully for encapsulated larvae (usually in inguinal region). Effectiveness is determined by comparing numbers of larvae in treated mice with numbers in untreated mice. Probit analysis is conducted with corrected percent kill data. Details of the test have been presented by Gingrich, R.E., etal., 1972, J. Econ. Entomol. 65(3): 742-5, and Drummond, R.O., and R. E. Gingrich, 1972, J. Econ. Entomol. 65(4): 1211-2. Recent resuhTwere presented by Drummond, R. O., 1976, Insect. Acaric. Tests 1: 117-8.