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Bovicola bovis collected from a naturally infested castrate bovine at the University of Wyoming Livestock Farm were transported to the laboratory at the University of Wyoming in Laramie. Appropriate serial dilutions of analytical standard (9.2%) trans-permethrin were prepared in reagent grade acetone. Acetone solutions were pipetted onto Whatman no. 1, 9.0 cm filter papers which were then allowed to air dry. Filter papers were placed into separate 100 × 15 mm Pyrex petri dish bottoms; 0.01 g of fresh, bovine skin scurf was distributed evenly over the surface of the filter paper. Randomly selected adult female B. bovis were placed directly on the treated filter paper in the petri dishes at the rate of ca. 25 ± 3 per dish. Polystyrene petri dish bottoms (100 × 20 mm) with air holes were inverted and placed over the filter papers to prevent louse escape and ensure continuous contact of lice with the upper surface of the treated paper. Petri dishes were placed in a growth chamber at 35.0°C and 75.5% RH. Mortality was determined after 24 h. Lice incapable of walking were considered dead. Data were analyzed by probit analysis using POLO-PC (LeOra Software, Berkely, Calif.).

Laboratory Study of Susceptibility of Cattle Biting Louse to Permethrin, 1990