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Two Microbial Insecticides Were Evaluated In The Laboratory With Field-Treated Tobacco Leaves Against Tomato Hornworms At The Research Station, Agriculture ! Canada, Delhi, Ontario. Tobacco Seedlings Of The Variety Candel Were Transplanted Into A Field Of Fox Loamy Sand On 28 May. Plots, 20 × 3.5 Ft Each, Were J Arranged In A Randomized Block Design With Two Replications. Each Plot Was A Single Row Of 10 Plants. Materials Were Applied To The Upper And Lower Surfaces J Of Tobacco Leaves Using A Knapsack Sprayer With An Adjustable Nozzle At A Rate Of 40 Gal Per Acre On 19 August. Test Insects Were 4Th- And 5Th-Instar Larvae J Of The Tomato Hornworm Collected From Tobacco Plants In An Untreated Field, And The Larvae, 10 For Each Replicate, Were Placed Individually In 24-Oz Wide mouth jars. Two hours after application of the insecticides, random samples of tobacco leaves were collected from each plant, taken to the laboratory and  placed in the jars with the larvae. Rearing was conducted at room temperature (72-74F) and 16 h photoperiod. Mortality was assessed daily for 4 days. Larvae that remained on the leaves and showed any reaction when gently touched were considered alive.

Efficacy of Microbial Insecticides on Tobacco Leaves for Tomato Hornworm Control, Ontario, Canada, 1985