Open AccessExpression of glucose transporter and glucose uptake in human oocytes and preimplantation embryos
Author(s) -
Mary DanGoor,
Shlomo Sasson,
Ana Davarashvili,
Miriam Almagor
Publication year - 1997
Publication title -
human reproduction
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.446
H-Index - 226
eISSN - 1460-2350
pISSN - 0268-1161
DOI - 10.1093/humrep/12.11.2508
Subject(s) - glucose transporter , embryo , cytochalasin b , oocyte , biology , glucose uptake , endocrinology , andrology , cytochalasin , medicine , blastocyst , human fertilization , embryogenesis , microbiology and biotechnology , cell , genetics , insulin , cytoskeleton
The expression of glucose transporters 1, 2, 3 and 4 was evaluated in human oocytes and polyploid preimplantation embryos. Only glucose transporter 1 (GLUT-1) isoform was detected in oocytes and in 2-12-cell stage embryos. Glucose uptake was markedly increased in embryos as compared to oocytes (19.7 +/- 3.4 pmol/min/embryo and 2.3 +/- 0.3 pmol/min/oocyte), and GLUT-1 was inhibited by cytochalasin B. These results suggest that, although GLUT-1 is expressed in human oocytes and throughout preimplantation development, its function in mediating the rise in glucose uptake is triggered following fertilization.
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