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Frataxin deficiency in Friedreich’s ataxia results from transcriptional downregulation of the  FXN  gene caused by expansion of the intronic trinucleotide guanine-adenine-adenine (GAA) repeats. We used multiple transcriptomic approaches to determine the molecular mechanism of transcription inhibition caused by long GAAs. We uncovered that transcription of  FXN  in patient cells is prematurely terminated upstream of the expanded repeats leading to the formation of a novel, truncated and stable RNA. This FXN early terminated transcript ( FXN-ett ) undergoes alternative, non-productive splicing and does not contribute to the synthesis of functional frataxin. The level the  FXN-ett  RNA directly correlates with the length of the longer of the two expanded GAA tracts. Targeting GAAs with antisense oligonucleotides or excision of the repeats eliminates the transcription impediment, diminishes expression of the aberrant  FXN-ett , while increasing levels of  FXN  mRNA and frataxin. Non-productive transcription may represent a common phenomenon and attractive therapeutic target in diseases caused by repeat-mediated transcription aberrations.

human molecular genetics

Premature transcription termination at the expanded GAA repeats and aberrant alternative polyadenylation contributes to the  Frataxin  transcriptional deficit in Friedreich’s ataxia